RNA Preparation and Purification
Zymo Research Corporation RNA Clean & Concentrator™-5, 200 preps
This 5 minute procedure is based on the use of a unique single-buffer system and Zymo-Spin™ technologythat allows for selective recovery of total RNA (> 17nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt).The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra-pure RNA. The RNA can be eluted from the Zymo-Spin™ IC Column in as little as ≥ 6 µl of RNase-free water. The highly concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.
Epoch Life Science Inc GenCatch™ Total RNA Extraction System (250 preps) Uniquely formulated buffers, reinforced columns with collection tubes, along with protocols.
Provides a simple, fast and cost-effective method to purify total RNA from various starting materials (such as cultured cells, tissues, bacteria, yeast, etc.). Total RNA with a molecular size greater than 200 nucleotides (which excludes smaller RNA such as 5S and 5.8S RNA, and tRNA) can be isolated. Silica membrane technology. Up to 65 ug yield. 200 ul elution volume. No phenol/chloroform extraction nor ethanol precipitation required. Instructions for sample type listed in protocol. Used by labs around the world. 18 month shelf life.
Research Products International Corp Direct-zol RNA MiniPrep Plus w/TRI Reagent, & Zymo-Spin IIICG Columns, Capped, 50 Preps
The Direct-zol RNA MiniPrep Plus Kits are RNA purification kits that provide a streamlined method for the purification of up to 100 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary.
Bioworld URINEEXFOLIATED CELL) RNA
URINE (EXFOLIATED CELL) RNA PURIFICATION KIT: For the rapid purification of total RNA (including microRNA) from exfoliated cells in urine. Isolate and detect total RNA from as little as 1 mL of urine; Provides high quality RNA for sensi...
Macherey-Nagel NucleoSpin RNA Plant, 50 preps
NucleoSpin RNA Plant (50) 50 preps for the isolation of RNA from plant - NucleoSpin RNA Plant Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase
New England Biolabs, Inc. 3'-O-Me-m7G(5')ppp(5')G RNA Cap Structure Analog – 5 umol
Blocking of the 3' -hydroxyl of m7G with 3' -0-Me assures that the capped transcripts are homogeneous. The 3' - hydroxyl of the non-methylated G is the only 3' - hydroxyl available for initiation. The 5' terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level. For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation, and a decrease in the formation of the initiation complex of mRNAs for protein synthesis. Certain prokaryotic mRNAs containing a 5 terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system. Also a cap requirement has been observed for splicing eukaryotic substrate RNAs. A method using E. coli RNA Polymerase primed with m7G(5' )ppp(5' )G or m7G(5' )ppp(5' )A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas.
Epoch Life Science Inc GenCatch™ Plant RNA Purification System (250 preps) Uniquely formulated buffers, shearing tubes, reinforced columns with collection tubes, along with protocols.
Provides a simple, fast and cost-effective method to purify total RNA from plant (and fungal) samples. Comes with shearing tubes for simple and fast homogenization and filtration of tissue. Total RNA with a molecular size greater than 200 nucleotides (which excludes smaller RNA such as 5S and 5.8S RNA, and tRNA) can be isolated. Silica membrane technology. Up to 100 ug yield. 50 ul elution volume. 100 mg sample size. Additional buffer provided for handling plant tissue that contains sticky secondary metabolites. RNA isolated can be used for a variety of molecular manipulations such as Northern blotting, RT-PCR, PolyA+ RNA selection, cDNA synthesis, Primer extension, in vitro translation, etc. No phenol/chloroform extraction nor ethanol precipitation required. Used by labs around the world. 18 month shelf life.
Research Products International Corp Direct-zol RNA MiniPrep w/Zymo-Spin IIC Columns, Capped, 200 Preps
The Direct-zol RNA MiniPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary.
Research Products International Corp Direct-zol RNA MicroPrep w/TRI Reagent, 50 Preps
The Direct-zol RNA MicroPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 µg (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis.
Macherey-Nagel NucleoSpin RNA Columns, 50 columns
NucleoSpin RNA Columns (50) binding columns for the isolation of RNA pack of 50
LAMDA BIOTECH INC Column-Pure RNA Mini-Preps
A spin column-based system designed for total RNA isolation from animal tissues and cultured cells. The kit employs selective RNA binding to silica membrane technology for nucleic acid purification.
New England Biolabs, Inc. Monarch® Total RNA Miniprep Kit - 50 preps
The Monarch Total RNA Miniprep Kit is a comprehensive solution for sample preservation, cell lysis, gDNA removal, and purification of total RNA from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. Additionally, tough-to-lyse samples, such as bacteria, yeast, and plant, can be processed with additional steps that enhance lysis. Total RNA, including viral RNA, can also be extracted from clinically relevant samples like saliva and swabs. Cleanup of enzymatic reactions or purification of RNA from TRIzol-extracted samples is also possible using this kit. Purified RNA is highly pure, has minimal residual gDNA and ranges in size from full-length rRNAs down to intact miRNAs. Additionally, differential binding conditions allow selective capture or exclusion of the sub-200 nucleotide RNA pool that includes miRNA, 5S rRNA, and tRNA. Purified RNA is suitable for downstream applications, such as RT-qPCR, RNA-seq, Northern blot analysis, etc.